2 MAIN UNITS AND DESCRIPTION OF CONTROLS
(2) Detection mode selector slider
Slider for selecting either two fluorescence signals or one fluorescence signal + transmitted
light 3 settings. This slider is placed at the A-section shown in Fig, (I).
• Slide'r at the pushed-in position
Select this mode when observing a transmitted light and / or a fluorescence with
emission wavelength shorter than 570nm, such as FITC, GFP, DiO, etc.
As shown in Fig. (I) on the following page, a double-sided mirror is engaged into the light
path.
Consequently, fluorescence light is reflected to the CHI (PMI). When the transmitted
light detector (option) is
attached,
the transmitted light is reflected via the optical filter
connection to the CH2 (PM2).
• Slider at the middle position
Select this mode when observing a double-stained specimen, such as FITC+PI, etc.
As shown in Fig. (II) on the following page, a beam splitting dichroic mirror is engaged
into the light
path.
The characteristics of this dichroic minor make it reflect light with
wavelengths below 570 nm and pass the longer wavelengths. Consequently, the
fluorescence light can be split into light for the 1CH (PMI) and the 2CH (PM2). With the
slider at this position, the transmitted light from the optical fiber connection is blocked.
•
Slider at the pulled-out position
Select this mode when observing a transmitted light image and / or fluorescence
specimen with emission wavelengths longer than 570 nm, such as PI, TRITC, etc.
As shown in Fig. (Ill) on the following page, nothing is engaged into the light
path.
Consequently, fluorescence light is directed to the CH2 (PM2). When the transmitted
light detector (option) is attached, the transmitted light is directed via the optical fiber
connection to the
CH1
(PMI).
2-3
