2 MAIN UNITS AND DESCRIPTION OF CONTROLS
• FITC emission tails longer than 570 nm. Accordingly, FITC fluorescence may be
detected on the CH2 where it overlaps the Pl fluorescence. (See the figure below.) The
problem may be remedied by cutting the FITC by adjusting the OFFSET on the
operation panel ([Acquire] panel). If not, It will be necessary to balance the excitation
light by reducing the intensity of the 488 nm excitation light used for exciting the FfFC.
When observing a double-labeled specimen, such as FITC+PI, attempt balancing the
intensities ofthe emissions by engaging the (3), (4), (5) filters in the described order.
CH,
A
°°N
AT25 A A \
(FITC overlapping the j \ 1 I 1
CH2 can be reduced by / Vl 1 \
attenuating the excitation 1 \ \
Y
\\
CH2
/~
/
^ /
/\ /
PI
1
400 500 600
wavelength / nm
700
2-6
