
2 MAIN UNITS AND DESCRIPTION OF CONTROLS
1
(3) Bamer filter slider
Up to two filters can be placed for both CHI and CH2. At the pushed-in position, the filter is
engaged into the light
path.
At the pulled-out position, it is disengaged.
* If erroneously placed at the middle position, no image will appear. Always stop at the
correct position.
(4) ND filter turret
There are five positions.
Transmission ratio: 0, 6, 20, 50, and 100%.
(5) Filter slider cover screws
Operated when the bamer filter slider or the detection mode selector slider is replaced.
When this screw is loosened, the covers can be pulled off. Then replace the sliders, retum the
cover to its original position, and tighten the screw again.
r Detection mode selector slider should be removed only, by a person who has had laser
L safety training, and only after tuming off the laser unit
(6) KrAr laser line filter tun-et
(Only attachable with the Kr and Ar laser combination)
Unit
writh
built-in 5-filter turret for selection in accordance with the 488 nm and 568 nm
excitation wavelength of the Kr
and
Ar laser.
(1)488:
The specimen is excited at 488 nm. Used for observation of single-stained
specimens, such as FITC.
(2) 568: The specimen is excited at 568 nm. Used for observation of single-stained
specimens, such as TRITC, PI.
(3) 568: The specimen is excited at 488 nm and 568 nm. However, the 488 nm output
488
°"'y'®
attenuated to 6% of the normal excitation light. Used for preventing the Pl
AT6 from eclipsing the FITC when observing double-stained specimens such as
FITC+PI.
(4) 568: The specimen is excited at 488 nm and 568 nm. However, the 488 nm output
488 only is attenuated to 25% of the normal excitation light Used for preventing the PI
AT25 from eclipsing the FITC when observing double-stained specimens such as
FITC+PI.
(5) 568: The specimen is excited at 488 nm and 568 nm. Used for observation of double-
+
^og Stained specimens, such as FITC+PI.
2-5