Section
1
Problem Countermeasures
^^^«^
6. Flare is visible.
7. Circular flares appear in
the center of the image.
8. Blun-ing is visible.
9. Images are dari< with
excessive noise.
10.
Poor reproduction of Z
motor positions.
11.
The Fluoview software
will not start.
12.
The [Acquire] panel is
not displayed.
•^m^amm
The cover glass ttiickness is
not suitable.
The specimen is
over-
stained.
The excitation wavelength
and absorption fllter type are
not matched.
An absorption filter has not
been inserted.
The focus is not correctly
adjusted.
The con-ect pinhole has not
been selected.
The dye color is too weak.
The pinhole is too small.
The photomultiplier HV
exceeds-800.
Scanning speed is too fast
The excitation light is too
weak.
The Z position slips because
the tension of ttie
microscope coarse
movement handle is too
light
The Fluoview software has
already been started.
Anotiier application software
is operating.
The control unit is not
recognized.
ftmmQounleTmeaswe"MMi
Use a cover glass witti a
thickness of 0.17mm.
Use an appropriate dye color
or increase the offset value.
Select an absorption fllter
that matches the excitation
wavelength.
Refer to Operation, 4-1-2.
Follow ttie insU^ctions in tiie
[Microscope Configuration]
window.
Re-focus using visual
observation.
Select the correct pinhole.
Set an appropriate dye
color.
Adjust to an appropriate
position.
Use an HV of less than -800.
If
dari<,
adjust the
gain.
Lower the scanning speed to
an appropriate speed or
integrate the images.
Change to a high
transmission ratio ND
filter.
Increase ttie tension by
tuming the adjustment ring
and adjusting until the
coarse movement handle
tension increases suitably.
Press the keyboard (Alt)
and fTab) keys to switch to
Fluoview.
Shut down the other
application software and
start the Fluoview
software.
Check Uie connection
between the computer and
contt-ol unit
Tum on tiie control unit
power supply.
Turn on the AUX2 switch of
the power controller.
;iManuai,iReference^'
— •
Operation,
4-1-3-10.
Operation,
4-1-2.
Hardware, 3-1-5
and 4.
Refer to ttie
microscope
instiojction manual.
Hardware, 2-1.
—
Hardware, 2-1.
Operation,
4-1-3-10.
Operation,
4-1-3-11.
Hardware, 2-1,
3-1-6.
Refer to the
microscope
instmction manual.
"
Operation,
2-1-1.
Inti-oduction to
Fluoview, 2-6-2.
1-3
