Section 1 Problem Countermeasures
Section
1
Problem Countermeasures
Due to Uie usage, the functions of the system sometimes are not fully effective, although there has been
no failure; tiierefore, when a problem occurs, refer to the following chart and apply the appropriate
countermeasures.
If the same phenomenon continues to occur after the countermeasures have been taken, contact your
local Olympus dealer.
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;Manual;Referencel
1.
No fluorescent light
image.
The laser is not oscillating.
Make sure ttie laser unit
power supply is tumed on
and ttie emission key is on.
Inttoduction to
Fluoview, 2-6-2.
The light path selector lever
is not set to the LSM light
path.
Set the light path selector
lever to the LSM light
path.
Hardware,
2-2,
3-1-2.
The visual observation
dichroic mirror and analyzer
are on the microscope side.
Remove the visual
observation dichroic mirror
and analyzer from the light
path.
The detector photomultiplier
voltage is low.
Use the PMT scale of the
[Acquire] panel to increase
the photomultiplier voltage.
Operation,
4-1-3-10.
The excitation light is weak.
Change to a high
transmission ratio ND fllter.
Hardware, 2-1,
3-1-6.
The fluorescent light dye
color and excitation
wavelength are not matched
(krypton/argon laser
combination).
Select a laser line filter that
matches the fluorescent light
dye color.
Refer to Operation, 4-1-2
Scan Unit Setting. Follow the
instructions in the
[Microscope Conflguration]
window.
Operation,
4-1-2.
Hardware, 3-1-7.
The fluorescent light dye
color and absorption filter
are not matched.
Select an absorption filter
that matches the fluorescent
light dye color.
Refer to Operation, 4-1-2
Scan Unit Setting, r Follow
the insti^ctions in the
[Microscope Configuration]
window.
Operation,
4-1-2.
Hardware, 3-1-5
and 4.
The confocal aperture is too
small.
Increase the aperture.
Hardware, 2-1.
The offset value is too large.
Lower the offset to an
appropriate value.
Operation,
4-1-3-10.
1-1
