Active
Siemens Critical Care Analysers SOP
Version:
2.00
Author
:
D Harrison
Doc Manager
:
D Harrison
Authorised by
:
Kath Ash
ton
Signature
: .
Ver Date
:
03/08/09
Do not photocopy this document
Page
7
of
24
Last printed:
5 August 2009
( David Harrison )
DMS Ref
:
Document in Microsoft Internet Exp
lorer
To install a PO2 or PCO2 sensor
As for installing glucose sensor except that PO
2
& PCO
2
are stored at room temperature so do not
require to be equil
ibrated to room temperature before use
Weekly Maintenance Procedures
Check the electrolyte level in each of the sensors, top up or replace as necessary
.
To install or service an electrolyte sensor (Na, K, Cl, Ca, pH)
These sensors and associated fill solutions are stored at room temperature.
i)
The box containing the sensor also contains a filling needle & a vial of filling solution.
This solution is interchangeable between Na, K, Cl, Ca sensors. The pH fill solution is unique to
the pH sensor.
ii)
Remove the centre
electrode by unscrewing from the body of the sensor and set to one side
taking
care not to touch the central pin. Fit the filling needle to the vial of electrode fill solution
& fill the body of the sensor f
rom the bottom to no more than 8
0% f
ull for K, Cl & Ca sensors
and 9
0% full for Na & pH sensors. Refit the central electrode & g
ently tap the sensor to ensure
that there are no bubbles on the membrane.
iii)
Open the electrode compartment. Release the clamp on the right.
iv)
Remove the old sensor and install new
v)
The response of the sensor can often be restored by replacing the sensor fill s
olution.
To do this, unscrew the centre
electrode & set to one side taking care not to touch the central
pin. Gently tap the old electrolyte out onto a tissue and replace with fresh as in step 2, refit the
central electrode and tap gently to
dislodge any bubbles that may be trapped on the membrane.
Refit sensor to the instrument and recalibrate
2)
Perform deproteinise and conditioning of electrodes.
i)
Reconstitute a vial of deproteiniser(D1b, pepsin powder)with a vial of D1a electrolyte mixtur
e.
Fill a 5ml syringe with the deproteiniser solution.
ii)
Press STATUS >
MAINTENANCE > DEPROTEINIZING THE SAMPLE PATH
N.B. if there are glucose or lactate sensors fitted you will be prompted to remove the glucose and lactate sensors
and install TB4 dumm
y electrodes and press OK to confirm BEFORE PROCEEDING WITH DEPROTEINISER.
FAILURE TO DO THIS WILL CAUSE IRREPARABLE DAMAGE TO THE SENSORS WHICH WILL
REQUIRE REPLACEMENT.
iii)
Insert syringe of deproteiniser to sample port and press ANALYSE
Remove syrin
ge when prompted. This takes 5 minutes.
iv)
Instrument will then prompt to ask for CONDITION??
Transfer the contents of one conditioner vial to a 5ml syringe and present to the analyser sample
port and press ANALYSE.
Remove syringe when prompted.
This
takes 5 minutes.
You will be prompted to remove the dummy electrodes and re-install glucose and lactate sensors and press
OK to confirm BEFORE PROCEEDING WITH CALIBRATION.
v)
Instrument will then prompt CALIBRATE?? press YES to calibrate.
The instrumen
t may require 2 or 3 calibrations after a clean/condition before all electrodes
re
-
stabilise.
Note that if any of the sensors have been replaced or disturbed then the analyser must be fully
calibrated and all levels of IQC measured before anyone is permi
tted access to analyse samples.
