A: Calibrating the Wavelength of the USB2000+
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7. Record the Intercept, as well as the First, Second, and Third Coefficients. Additionally, look at
the value for R squared. It should be very close to 1. If not, you have most likely assigned one of
your wavelengths incorrectly.
Keep these values at hand.
Saving the New Calibration Coefficients: USB
Mode
Ocean Optics programs wavelength calibration coefficients unique to each USB2000+ onto an EEPROM
memory chip in the USB2000+.
You can overwrite old calibration coefficients on the EEPROM if you are using the USB2000+ via the
USB port.
► Procedure
To save wavelength calibration coefficients using the USB mode, perform the following steps:
1. Ensure that the USB2000+ is connected to the computer and that you have closed all other
applications.
2. Point your browser to
http://www.oceanoptics.com/technical/softwaredownloads.asp and
scroll down to
Microcode. Select USB EEPROM Programmer.
3. Save the setup file to your computer.
4. Run the
Setup.exe file to install the software. The Welcome screen appears.
5. Click the
Next button. The Destination Location screen appears.
6. Accept the default installation location, or click the
Browse button to specify a directory. Then,
click the
Next button. The Program Manager Group screen appears.
7. Click the
Next button. The Start Installation screen appears.
8. Click the
Next button to begin the installation. Once the installation finishes, the Installation
Complete
screen appears.
9. Click the
Finish button and reboot the computer when prompted.
10. Navigate to the
USB EEPROM Programmer from the Start menu and run the software.
11. Click on the desired USB2000+ device displayed in the left pane of the
USB Programmer
screen.
12. Double-click on each of the calibration coefficients displayed in the right pane of the USB
Programmer screen and enter the new values acquired in Steps 5 and 6 of the
Calibrating the
Wavelength of the Spectrometer section in this appendix.
13. Repeat Step 12 for all of the new values.
14. Click on the
Save All Values button to save the information, and then Exit the USB Programmer
software.
The new wavelength calibration coefficients are now loaded onto the EEPROM memory chip on the
USB2000+.
