Linear Variable Filters
Linear Variable Filters 3
Configuring the Cuvette Adapter (LVF-CUVADPT)
With the LVF-CUVADPT attached, you can still access all openings and setscrews on the original cuvette holder.
To use the Linear Variable Filters with any of the available Ocean Optics 1 cm pathlength cuvette holders, follow
the steps below:
1. Remove the collimating lenses or mirrored screw plugs from the cuvette holder so that the LVF-
CUVADPT can fit over the existing cuvette holder structure.
2. Align the groove in the filter holder of the existing cuvette holder with the filter groove on the underside of
the LVF-CUVADPT. See the illustration below.
Side View – LVF-CUVADPT connection to an Ocean Optics CUV-ALL-UV 4-way cuvette holder
3. Slide the LVF-CUVADPT down over the existing cuvette holder.
4. Reattach the screw-in attachments through the holes in the sides of the LVF-CUVADPT, securing the unit
to the cuvette holder.
You have now properly attached the Cuvette Adapter to the cuvette holder.
Configuring the Inline Filter Holder (FHS-LVF)
Designed for use with reflection measurements, the FHS-LVF Inline Filter Holder enables you to easily position
the Linear Variable Filters in your optical path. The FHS-LVF features collimating lenses on each optical input that
focus the light source used in your experiments into a tight, collimated beam.
You need an additional optical patch cord to use the Inline Filter Holder with a reflection probe.
Follow the instructions below to configure the Inline Filter Holder for use with an Ocean Optics reflection probe for
filtering on the excitation side of the fluorescence measurement:
1. Connect an optical patch cord from your light source to one side of the Inline Filter Holder.
2. Connect the illumination leg of the reflection probe (6 fibers) to the other side of the Inline Filter Holder.
3. Connect the detection leg of the reflection probe (1 fiber) to the SMA 905 connector of your spectrometer.
4. Open OOIBase32 and begin to acquire data in scope mode using a white standard.
5. Adjust the collimation lenses on the sides of the Inline Filter Holder until you achieve maximum signal
strength. If necessary, refer to the Collimating Lenses operating instructions located here:
http://www.oceanoptics.com/technical/collimatinglenses.pdf
You have now configured the Inline Filter Holder for use.
