Section 2 System Overview
2-7-2 Transmitted Light Observation Procedure
start the system.
Prepare the system
(tum on the power supply).
Introduction to Fluoview (2-6)
start
the
fluoview softvyrare.
Operatton (2-1-1)
.
Look thraugti
the eyd(jto0^l0ns and
focus on
ttie specimen.
" Select
the
^; path for
100%
for ^
binocular
tube
and
focus
on the specimen.
:
H*dware
•Set to the LSM light
path.
Set to 1t)e LSM light
path.
HardWWe (3-2-3)
I f « Y.|»« I
Set Ihe scan unit.
Remove the microscope caps and
filters from the light
path.
[
Operate the dialection mode setting
knob and $et the channel.
Hardware (3-2-4)
Insert
the
absoi|7lJqc^ filter into the light
path.
Hardware (3-2-5)
Operate the ND filter turret and
sef0<:^
^ ND filter
(100%,
50%.
20%, 6%).
H^dw^re
(3-2-6)
^Replace with a high transmis^i;^ rate^
ND filter (100%, 50%, 20%,^5%).
Hardware '
(3-2-6)
Read in an image.
Operation (3-1)
• -w-^
*.j*
.•
<
Set the Ot)idervation
con<itlons.
Scan once dftepeatedly.
If the image is not
displayed,
adjust
In accordance with
the relevant
procedure.
%
m
The
image
is \
displayed l(t|he^
software [LjveJ »
panel.
*
if;dr)im«S«)9
Adjust the PMT voltage* J
If an iinei^e i$
tSsplayed,'
Stop scann^g.
Shut down the system.
Shut down the Fluoview softv\^are.
Operation (2-1-2)
Shtltdown the system.
(Turn off the power supply.)
2-16
